Using The CD45RA For Research

The CD45 antibody is a membrane sialoglycoprotein that includes three exons that encode the peptide segments. You can create various isoforms with differential splicing of the exons. The CD45RA is just one single chain of glycoprotein, and it is expressed on about 40 to 50 percent of all peripheral CD4+T-cells, as well as 50 percent of B-cells, leukemic B-cell lines, and peripheral CD8+ t-cells. Any T-cells that express this antibody can be used to define complementary and predominantly non-overlapping resting peripheral T-cells.

More About It

The CD45RA antibody is only designed for research, and it does have a clone called SPM504. The immunogen is the stimulated human leucocytes, and the isotype is the IgG2a. It has no known epitope and a molecular weight between 205 and 220kDa. It has been tested on humans and can be used for Immunohistochemistry applications.

IHC Application Procedure

You can use paraffin-embedded or Formalin-fixed tissues for this antibody. Deparaffinized slides must be utilized, and you can clean them with xylene alternatives, xylene, or graded alcohols.

When using the concentrated format, you’ll want to dilute the antibody using a ratio of 1:200. However, dilutions are an estimation. You should read the instructions and follow the protocols provide for your testing needs. There is no retrieval method necessary, but you will need to incubate the antibody for 30 minutes while at room temperature. Make sure to wash the slides between steps and detect your antibody as per the instructions. The positive control is the tonsil with cellular localization occurring in the membrane.

You can find a 7.0 ml pre-diluted formula for purchase, but you can also find concentrated formats of 0.1, 1.0, and 0.5 ml.

The CD45RA can be used to define a variety of peripheral T-cells. Visit Spring BioScience for purchasing options.


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