Learn More About Using The FOXP3 Antibody

The FOXP3 antibody is a member of the forkhead/winged-helix family with regards to transcriptional regulators and is also a 431 amino acid protein. It is highly conserved across all mammals and is essential for regular immune homeostasis. It is stably expressed at high levels in CD35+CD4 positive regulatory T-cells, but at lower levels in CD4 positive/CD35 negative cells. It is completely absent in CD4 negative-CD8 positive T-cells. It could be a master regulatory gene and specific market for regulatory T-cells.

More Information

The FOXP3 antibody comes in polyclonal and monoclonal form. The Polyclonal version has no available clone, and the monoclonal version has a clone of SP97. The immunogen is the synthetic peptide that corresponds to the C-terminus of the same protein in humans. The isotype is the Rabbit IgG, and it has an undetermined epitope. The molecular weight is 50 kDa.

Applications

The FOXP3 antibody is designed for use with Immunohistochemistry applications. To begin, you should use Formalin-fixed or paraffin-embedded tissues, as well as deparaffinized slides. You’ll find a variety of sizes available for purchase and most of them are concentrated, which means you will need to dilute them using a ratio of 1:100. If that is the ratio you require, you can also find a pre-diluted formula. However, some protocols and methods will require different dilutions, so pay close attention to what your system says.

To retrieve the antigen, you should boil the tissue section for ten minutes in an EDTA buffer with a pH of 8.0. Afterward, allow the concoction to cool for 20 minutes. Then, you should incubate at room temperature for 30 minutes.

The positive control is the tonsil with cellular localization occurring in the nucleus.

The FOXP3 antibody can be used to mark a variety of things in research. Visit Spring Bioscience today to find out more.

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